The method of tissue optical clearing (TOC) is based on a temporary and significant reduction in light scattering in tissues and leads to the creation of new virtual and expansion of known transparency windows of various tissues in a wide range of wavelengths from deep UV to THz waves. It is an effective means of improving the quality of optical images of tissues and organs, and has great prospects for in-depth diagnostics and effective photodynamic and photothermal therapy [1]. The lecture summarizes the latest advances in the development of the TOC method for solving problems of optical imaging and therapy. In addition to increasing optical transparency, studying the kinetic properties of tissue transparency under the influence of probe molecules of optical clearing agents allows one to reliably differentiate between healthy and pathological tissues and quantify the mechanisms of drug delivery, as well as combine optical technologies with traditional imaging methods such as ultrasound, CT and MRI.

The talk reviews recent advances in area of cell modification by number of colloidal contracts. Impregnation of cells might go by different mechanism and results in internalisation of functional particles and capsules. The later can serve as intracellular exploration tool to interrogate with cell interior, natural transporters as system with autoimmune cells bearing encapsulated cargo as drug to designated recruiting side in vivo and photoconvertible particles whose combination facilitates individual cell labelling and tracing single cells in complex multicellular systems.

The development of micro and nanomanufacturing technology is a core issue in the scale-up manufacturing and application of nanomaterials to devices. We utilize the printing templates to precisely control droplet formation, assembly, and patterning of functional materials, achieving high-precision three-dimensional micro and nano structures with an accuracy of 100 nm. By introducing a droplet self-shaping strategy driven by minimizing surface energy into the printing, we have developed a structure-function integrated printing method to fabricate wearable electronics and smart optoelectronics. We have printed photonic resonance structures with single-nanoparticle precision, and verified the critical size for the scattering-diffraction transition in micro/nano structures. When viruses and other biological particles are recognized on the surface of structure, scattering resonance occurs with near-field enhancement, which significantly changes the color of scattered light. This enables rapid identification of target viruses directly from serum or sputum within 15 minutes.

The report will consider the basic principles of explaining the possibility of using magnetic nanoparticles in areas such as targeted drug delivery, magnetic hyperthermia and magnetomechanics, as well as MRI. Using our own work as an example, we will demonstrate the importance of selecting synthesis parameters for obtaining magnetic nanoparticles with a given structure and morphology in order to maximize efficiency in each application.

Aggregation-induced emission luminogens (AIEgens) represent a unique class of luminescent materialsexhibiting enhanced emission upon aggregation, unlike conventional fluorophores prone to quenching. This property minimizes background fluorescence and enables robust emission in the aggregated state,making them highly desirable for bioimaging and organic optoelectronic devices. The ongoing development of AIEgens with precisely controlled properties holds particular promise for nanomedicineresearch. By incorporating AIEgens into nanoparticles, researchers can gain valuable insights into particle biodistribution, cellular trafficking, and degradation in biological systems, ultimatelycontributing to advancements in nanoparticle design and understanding their impact on living organisms. This presentation will showcase the use of AIEgens in novel albumin nanoparticles designedfor endosomal degradation and track microplastic fate in liver cells and zebrafish models.

The report will present approaches to camouflaging inorganic nanoparticles with biological membranes for treating oncological diseases. Data on creating biological membranes with targeted properties based on human cells, the features of camouflage, the completeness of the coating, and the options for using this technology to solve the problems of targeted nanoparticle delivery, increasing bioavailability, reducing toxicity, and intracellular nanoparticle delivery will be considered. Results on the effect of biocamouflage on spectral properties of upconversion nanoparticles and their activity in vitro and in vivo will also be demonstrated.

This proposal focuses on the requirements of motion detection in dynamic environments and multi-analyte sensing in exercise monitoring. By leveraging the excellent optical properties of photonic crystals, we have designed a stretchable photonic crystal sensing chip to capture abundant optical information during the stretching process. This makes it possible to adapt to the sensitive discrimination and analysis of clinical complex samples during exercise. The specific innovations are as follows: 1) Principal innovation: We will explore the structural color behavior during the stretching process of photonic crystals to establish the structure-activity relationship between photonic crystal deformation and structural color, as well as the slow photon effect (fluorescence enhancement). This will lead to the pioneering proposal of a scientific model of elastic structural photonics. 2) Material innovation: We will investigate the structure-activity relationship between the material elastic modulus and the photonic crystal elastic nanostructures. Additionally, we will elucidate the hydrogel molecular mechanism of the Hofmeister effect to adjust the elastic modulus of the stretchable photonic crystal, enabling the stretchable photonic crystal chip to adapt to various dynamic analysis environments. 3) Application innovation: We will develop clinical rapid detection chips and methods for multiple biomarkers, sweat, and blood samples in dynamic environments, promoting innovative clinical applications such as exercise fatigue, chronic disease diagnosis, and daily health monitoring. Based on the principal innovation of elastic structural photonics, we aim to advance the clinical application of complex sample discriminative analysis and dynamic environment detection in exercise monitoring and analysis.

Cancer remains one of the leading causes of death in the world. Despite the high efficiency ofmodern methods of tumor therapy, such as surgery, chemotherapy, radiation therapy, and photodynamic therapy, they are not free from shortcomings. Plasmonic photothermal therapy(PPT) using local hyperthermia induced by nanoparticles has recently been extensively explored in tumor treatment. However, the current PPT status is limited to laboratory experiments,academic papers, and only a few preclinical studies. This lecture discusses the current challenges and prospects of plasmonic nanomedicine and our achievements in developing possibleapproaches to improve the effectiveness and selectivity of PPT.